FIGURE 3.

Bacterial burden in the body trunk and intestine of L. monocytogenes EGDe infected germ-free fish was much higher than that of the strain M7 or L. innocua infected ones. The fish (50 per strain) were immersion-infected at 5 dpf and sacrificed at 24 hpi. The body trunk and intestine samples of each strain were divided into 10 subgroups, each having five fish larvae to be homogenized and resuspended in 100 μl of PBS, from which a volume of 10-μl suspension was spotted in triplicate on the PALCAM agar plates for bacterial enumeration. Data were expressed as log₁₀ CFU per five fish.