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. 2015 Apr 29;5:9613. doi: 10.1038/srep09613

Figure 5. TOFA inhibited lipid accumulation and restored the DC function.

Figure 5

In the presence of TOFA, immature DCs were co-cultured with serum from mice with radiation-induced thymic lymphomas for 60 h, DCs were stained with BODIPY493/503 for lipid content analysis (A). In the presence or absence of TOFA, the expression of CD80, CD86, Ia, CD40 and CCR7 of serum treated DCs was analyzed by FACS analysis (B). Immature DCs were cocultured with serum from mice with radiation-induced thymic lymphomas for 60 h in the presence of TOFA, harvested and then further co-cultured with CD4+ T cells from DO11.10 OVA323–339-specific (TCR-transgenic × C57BL/6) F1 mice for 5 days in the presence of OVA. Finally, the number of viable CD4+ T cells (CD4+7-AAD−) was detected by FACS analysis(C). All data are presented as the mean ± s.d. of three independent experiments. *P < 0.05.