Figure 6. High-fat diet increased lymphoma incidence and reduced the survival rate of radiation-treated mice.

Twenty C57B/L6 mice were fed a high fat diet (HFD) for 15 weeks, then the thymus was isolated and weighted, 20 mice fed with normal fat diet (NFD) served as the control (A). Single cells were prepared, and stained with Ab to CD11c. The CD11c positive cells were analyzed by FACS analysis (B). Twenty mice with radiation-induced thymic lymphomas mice were fed with the HFD or NFD, and the weight of the thymus was evaluated. 20 WT mice were used as the control (C). Twenty mice with radiation-induced thymic lymphomas were fed the HFD or NFD, and the percentage of DCs in thymus was examined by FACS analysis. Twenty WT were used as control (D). Twenty C57B/L6 mice were fed with the HFD for 15 weeks, the other 20 mice were fed the NFD as the control. In the last four weeks, mice received radiation treatment as described in the method. All mice were then euthanized to assess lymphoma incidence (E). The survival status of these treated mice were recorded after radiation treatment. The Kaplan-Meier curves were drawn according to the fat diet (F). All data are presented as the mean ± s.d. of three independent experiments. *P < 0.05.