Fig. 1.
Effect of androgen treatment on Skp2 expression and cell proliferation. A: LNCaP cells were grown in 10% CSS for 24 h and then incubated for an additional 48 h with the given concentrations of R1881. Western blots were performed with antibodies to Skp2, p27KIP1 and Erk2 (loading control). B: LNCaP cells were grown in 10% CSS for 24 h and then either treated with vehicle (mock) or R1881 (1 nM) in the presence or absence of bicalutamide (10 μM). At indicated time points, cells were harvested and lysed for western blot analysis with antibodies indicated. C: LNCaP cells were treated as in (A) and harvested for FACS analysis. The percentages of cells in S phase shown are the average of three independent experiments. D: Growth of LNCaP cells treated with different concentrations of R1881 was measured by the MTS assay as described in Materials and Methods. Data are means + s.d. from experiments with five replicates (n = 5).