Fig. 4.

Potential role of E2F1 and pocket proteins in regulation of Skp2 expression. A: Effect of E2F1 overexpression on Skp2 expression in PCa cells. LNCaP cells were transfected with the empty vector pcDNA3.1 or E2F1. At 36 h after transfection, cells were harvested and lysed for Western blot analysis antibodies as indicated. B: Potential role of pocket proteins in androgenic repression of Skp2 expression in PCa cells. LNCaP cells were transfected the empty vector pCI, wild-type and mutated E1A (RG2). At 24 h after transfection, the media was changed to CSS and then R1881 or vehicle was added. After 48 h cells were harvested and protein expression of Skp2, E1A, PSA and Erk2 (loading control) was determined by Western blot.