Table 3.
Microcytotoxicity assay results for the presence of anti-ELA-A2 antibodies in recipient sera
| Recipient serum dilution | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Recipient horse | Day | Neat | 1:2 | 1:4 | 1:8 | 1:16 | 1:32 | 1:64 | 1:128 | 1:256 | 1:512 | 1:1,024 | 1:2,048 |
| A | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 21 | 0 | 0 | 0 | <10 | <10 | <10 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 28 | 20 | 30 | 35 | 30 | 30 | 15 | 10 | 10 | 10 | 10 | 10 | 10 |
| A | 35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | Reinjection = 36 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 42 | 60 | <10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 49 | 90 | <10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| A | 56 | 50 | <10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 14 | 80 | 80 | 100 | 90 | 10 | 1 | 15 | 0 | 0 | 0 | 0 | 0 |
| B | 21 | 100 | 100 | 100 | 100 | 80 | 25 | 35 | 20 | 10 | 10 | 5 | 0 |
| B | 28 | 45 | 75 | 90 | 80 | 80 | 45 | 15 | 15 | 15 | 25 | 20 | 25 |
| B | 35 | 80 | 90 | 100 | 50 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 42 | 80 | 100 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 49 | 80 | 90 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| B | 56 | 80 | 30 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| C | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| C | 7 | 45 | 95 | 80 | 15 | 15 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| C | 14 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 80 | 30 |
| C | 21 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 85 | 50 |
| C | 28 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 55 | 25 | 15 |
| C | 35 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | 0 | 0 | 0 |
| C | 42 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 50 | 0 | 0 | 0 | 0 |
| C | 49 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | 0 | 0 | 0 | 0 |
| C | 56 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 21 | 0 | 0 | 10 | 25 | 25 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 28 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| D | 35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | Reinjection = 36 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 42 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 49 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| D | 56 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| E | 14 | 65 | 70 | 100 | 50 | 30 | 15 | 15 | 15 | 10 | 10 | 10 | 10 |
| E | 21 | 50 | 60 | 95 | 50 | 15 | 15 | 15 | 15 | 10 | 10 | 10 | 10 |
| E | 28 | 25 | 90 | 35 | 15 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| E | 35 | 0 | 80 | 50 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E | 42 | 25 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E | 49 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| E | 56 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 14 | 25 | 25 | <10 | <10 | <10 | <10 | 10 | 15 | 20 | 20 | 20 | 20 |
| F | 21 | 90 | 95 | 95 | 100 | 100 | 90 | 65 | 50 | 25 | 15 | 20 | 10 |
| F | 28 | 40 | 65 | 95 | 90 | 80 | 25 | 20 | 20 | 15 | 10 | 15 | 15 |
| F | 35 | 40 | 80 | 90 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 42 | 24 | 80 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 49 | 40 | 90 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| F | 56 | 20 | 80 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 42 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 49 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control (Horse 2) | 56 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Internal control (A2 – sera) | Days 0 to 28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Internal control (A2 – sera) | Days 35 to 56 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Internal control (A2 + sera) | Days 0 to 28 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Internal control (A2 + sera) | Days 35 to 56 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Data reported as the percentage death of ELA-A2 donor leukocytes. For each time point, the value in italics is the cytotoxic antibody titer (the highest dilution of each antisera that resulted in killing of at least 80% of the donor leukocytes as reported in Figure 2). ELA-A2, equine leukocyte antigen haplotype.