Figure 4.
The interaction between VpuC and β-TrCP is important for efficient CXCR4 downregulation. (a) A representative FACS plot of independent SupT1 infections showing CXCR4 surface expression 1 week after transduction. Similar results were observed in Jurkat cells. (b) Total cytoplasmic lysates were analyzed by western blotting with a polyclonal anti-CXCR4. NIH3T3 cells stably expressing human CXCR4 were used as a positive control. Similar results were observed in two independent experiments.