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. 2015 Feb 11;6(6):3669–3679. doi: 10.18632/oncotarget.2898

Figure 4. Notch3 plays a crucial role in HCC progression by interacting with β-catenin.

Figure 4

The Notch3 expression profiles of several HCC cell lines were screened by western blotting (A); siRNA oligos were applied to knockdown Notch3 (B); β-catenin and Nanog were detected via western blotting after Notch3 downregulation by transfection with a siRNA in HepG2 and QGY7701 cells (C, D); The knockdown of Notch3 enhanced the LiCl-mediated activation of the β-catenin pathway and reduced Nanog expression in the QGY7701 cells (E); LiCl-mediated β-catenin activation directly negatively regulated the Nanog protein level in QGY7701 cells (F); A promoter-activity reporter assay was performed to measure the β-catenin promoter activity regulated by Notch3 (G, si-NC = si-negative control, si-1# = si-Notch3–1, si-4# = si-Notch3–4).