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. 2015 Jan 21;6(6):3797–3810. doi: 10.18632/oncotarget.2902

Figure 5. Expression profiling of V-ATPase ‘a’ subunit isoforms in ovarian cancer.

Figure 5

Real time PCR was used to quantify the relative mRNA amounts of V-ATPase V0‘a’ isoforms (V0a1/V0a3/V0a4) in ovarian cancer cell lines. The ovarian cancer cells exhibited higher mRNA levels of (A) V0a1 and (B) V0a3 isoforms in ovarian cancer cell lines compared to normal control epithelial cells of ovary. (C) V0a4 isoform levels were very low and variable in different cell lines. The Ct values were normalized against the Ct values obtained for GAPDH from the same preparation. The data are provided as Mean ± SD from 3 independent experiments. (***p < 0.0001, **p < 0.001, *p < 0.05). (D) V0a1 (green) exhibited some co-association with plasma membrane marker, pan-cadherin (red), merged image shows yellow area, scale bar 5 μm. (E) V0a3 (green) did not exhibit co-association with plasma membrane marker, pan-cadherin. Original magnification- X 600, scale bar 5 μm.