Skip to main content
. 2015 Feb 2;6(6):3840–3847. doi: 10.18632/oncotarget.2927

Figure 1. MicroRNA-153 mimics promotes β-catenin signaling in HCC cells.

Figure 1

(A) HepG2 cells transiently transfected with the TopFlash–FopFlash and the indicated MicroRNAs for 36 hr. Luciferase values were measured using the Dual-Luciferase Reporter Assay System and renilla luciferase gene was used to normalize the transfection efficiency. (B–C) Relative mRNA (B) and protein (C) levels of Cyclin A, Cyclin D1, Cyclin E, c-myc and p21 were determined in HepG2 cells transfected with miR-153 mimics (MM) or negative controls (NC) for 24 or 48 hr, respectively. (D) Cell-cycle analysis of HepG2 cells transfected with NC or miR-153 mimics. Cells were stained with propidium iodide and analyzed by flow cytometry. (E–F) Cell proliferation (E) and colony formation (F) assays by transfection of NC or miR-153 mimics.