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. 2015 Feb 2;6(6):3840–3847. doi: 10.18632/oncotarget.2927

Figure 2. MicroRNA-153 antisense inhibits β-catenin signaling.

Figure 2

(A) HepG2 cells transiently transfected with the TopFlash–FopFlash and miR-153 antisense (AS) or NC for 36 hr. (B–C) Relative mRNA (B) and protein (C) levels of Cyclin A, Cyclin D1, Cyclin E, c-myc and p21 were determined in HepG2 cells transfected with miR-153 AS or negative controls (NC) for 24 or 48 hr, respectively. (D) Cell-cycle analysis of HepG2 cells transfected with NC or miR-153 AS. (E–F) Cell proliferation (E) and colony formation (F) assays by transfection of NC or miR-153 AS.