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. 2015 Feb 24;6(6):3918–3931. doi: 10.18632/oncotarget.2928

Figure 2. Induction of miR-199a-5p by PPIX.

Figure 2

(A) A list of miRNA candidates targeting HIF1A. The miRNAs were found using TargetScan 6.1 and miRanda. (B) The effect of PPIX on the expression of miRNAs targeting HIF1A. The levels of miRNAs were determined by qRT-PCR assays in SK-Hep1 cells treated with 3 μM PPIX. (C) Sequence motifs of the 3′-UTR of HIF1A match seed sequences of candidate miRNAs. (D) The levels of miR-199a-5p after PPIX treatment in mesenchymal HCC cells. qRT-PCR assays for miR-199a-5p transcripts in SNU878 or SNU449 cells. For B and D, the data represent the mean ± S.E. from 3 or 4 different independent experiments (significantly different from vehicle-treated control, *P < 0.05; **P < 0.01; N.S., not significant).