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. 2015 Feb 19;6(6):3932–3946. doi: 10.18632/oncotarget.2846

Figure 4. Effects of HRG on adhesive abilities of luminal HER2+ breast cancer cell lines.

Figure 4

(A) HRG increases adhesion of MDA-MB-361 and SKBr3 cells to collagen-I. Serum-starved cells were treated with HRG for 48 h, allowed to adhere to collagen-I-coated dishes for 15 min then adhesion potential was determined using optical density measurements of treated versus untreated controls. *p = 0.05–0.01, **p = 0.01–0.001 (2-tailed, unpaired student's t-tests). HRG increases expression of ICAM-1 RNA (B) and protein (C) in MDA-MB-361 and SKBr3 cells. Cells were treated as above, then total RNA or protein were prepared for quantitative real-time PCR or Western blot of ICAM-1 expression respectively (HPRT1 or α-tubulin loading controls, respectively). **p = 0.001–0.0001; ***p < 0.0001 (2-tailed, unpaired student's t-tests). FC, fold-change.