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. 2015 Jan 9;6(6):4080–4096. doi: 10.18632/oncotarget.2977

Figure 8. TRAF4 promotes the proliferation of breast cancer cell mostly through mTOR/p70s6k/S6 signaling.

Figure 8

(A) Western blot of wild type and TRAF4 mutants transfection efficiency in MCF7 cells. (B, E) TRAF4 promotes the proliferation of MCF7 and Zinc fingers domain was critical for it. MTT assay and colony formation assay were performed to analysis the proliferation of MCF7 after it was transfected with WT-TRAF4, ΔR, ΔT and ΔZn mutants vectors. Overexpression of WT-TRAF4, ΔR, and ΔT mutants all significantly promoted the proliferation of MCF7, but overexpression of ΔZn mutant had no obviously or just a little effection on proliferation of MCF7. (C) Western blot of TRAF4 transfection and p70s6k depletion efficiency in MCF7 cells. (D, F) TRAF4 promotes the proliferation of breast cancer cell mostly through p70s6k/S6 signaling in MCF7 cells. MTT assay and colony formation assay were performed to analysis the proliferation of MCF7. Knockdown p70s6k significantly but not absolutly inhibited the promoting effect of TRAF4 on proliferation of MCF7. Data represent mean ± SD (n = 3). (*p < 0.05).