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. 2015 Feb 14;6(6):4126–4143. doi: 10.18632/oncotarget.2798

Figure 4. Effects of MC3181 on human melanoma cell lines.

Figure 4

A375 (left panels) and SK23-MEL (right panels) cells were treated with equitoxic concentrations of MC3181 (10 μM and 7 μM, respectively). (A) MC3181 treatment (-■-) induced a sustained growth inhibition in both A375 and SK23-MEL cell lines, while untreated cells (-▲-) were in active proliferation. (B) Cell cycle analysis of A375 and SK23-MEL cells exposed to MC3181 for 48 hrs. (C) Time-dependent caspase-3 activation in A375 and SK23-MEL cells treated with MC3181. (D) Time-dependent phospho-activation of JNK in A375 and SK23-MEL cells treated with MC3181; actin was used as loading control. Densitometric analysis revealed a sustained and prolonged activation of JNK in the A375 cell line, while phosphorylation of JNK was early and transient in SK23-MEL cells. Data represent means ± SD of four independent experiments; (*) P < 0.05, (**) P < 0.005 and (***) P < 0.0005.