Skip to main content
. 2015 Jan 13;6(6):4214–4225. doi: 10.18632/oncotarget.2929

Figure 1. Glycolytic metabolism stimulates open global chromatin structure.

Figure 1

(A) A549 cells treated with glycolysis inhibitor 2-DG or transfected with siRNA targeting key glycolysis enzymes HK1, PKM were digested with increasing concentration of Micrococcal nuclease (MNase). Genomic DNA was analyzed with agarose gel electrophoresis. (B) Immunofluorescent staining with heterochromatin marker HP1α and H3K9me3 antibody in A549 cells treated with control, 2-DG or transfected with siRNA targeting HK1, PKM. Scale bar is 5 μm. (C) A549 cells treated with 2-DG or transfected with siRNA targeting HK1, PKM were fixed and analyzed by transmission electron microscopy. Arrows indicate heterochromatic regions. Scale bar is 5 μm.