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. 2015 Jan 13;6(6):4214–4225. doi: 10.18632/oncotarget.2929

Figure 2. Glycolytic metabolism stimulates global histone acetylation.

Figure 2

(A) A panel of human cell lines (293, A549, HCT116, MCF7, MCF10A, PC-3) and mouse embryonic fibroblast (MEF) were treated with 2-DG (10 mM) for 24 hour. The cells were analyzed by western blot with a lysine acetylation specific antibody. HSP90 and β-actin blots serve as loading controls. (B) Individual histone acetylation was probed with specific histone acetylation antibodies. (C) A549 cells transfected with siRNA targeting HK1, PFKL, PKM were analyzed by western blot with the lysine acetylation specific antibody. HSP90 and β-actin blots serve as loading controls. (D) A549 cells treated with 2-DG, or transfected with siRNA targeting HK1, PKM were subject to immunofluorescence analysis with histone H3K27 acetylation specific antibody. Scale bar is 5 μm.