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. 2015 Jan 19;6(6):4274–4285. doi: 10.18632/oncotarget.2760

Figure 3. Immunoreactivity of fractions eluted from Sepharose CL-2B.

Figure 3

(A) Capan-1 cell culture supernatant was separated on a Sepharose CL2B column with the eluted fractions analyzed by hPAM4 and α-MUC1. (B) The void-volume (Vo) fractions of Capan-1 reacted positively with three anti-MUC5AC antibodies (45M1, 1-13M1 and H-160), but not with 2Q445, which recognizes the unglycosylated tandem repeat region of MUC5AC. (C) The Capan-1 void-volume peak, following capture by 2-11M1, could be detected directly by HRP-hPAM4, or indirectly by biotin-45M1 plus SA-HRP.