Figure 6. Rapamycin inhibits phosphorylation of mSin1 independently of mTORC2.

Rh30 cells, infected with lentiviral shRNAs to GFP and rictor (A) or GFP and Akt (B) respectively, were serum-starved for 24 h. The cells were then pretreated with or without rapamycin (Rapa, 100 ng/ml) for 2 h, and further stimulated with or without IGF-1 (10 ng/ml) for 10 h, followed by Western blotting with indicated antibodies.