Figure 2. GHSR methylation degree in all samples analyzed.

Methylation of CpG GHSR_–1620 was analysed by bisulfite pyrosequencing. Percentage of methylation (vertical axis) is plotted against the sample type (horizontal axis): tumor (T) and normal-appearing tissue (N). Each circle indicates the methylation degree of a particular specimen. Horizontal bars denote the median methylation level for the cancer samples or the normal controls, respectively. For CLL, B cells of healthy donors were employed as control. Data are shown for the pooled specimens of seven malignancies as well as separately for each tumor type. The number of samples and P-values (Wilcoxon test) are additionally shown. Average of methylation levels of the 4 exonic CpGs (marked in Figure 1) is used for colorectal cancers.