Figure 3.

Human embryonic stem cell (hESC)-derived radial glia (RG) generate layer-specific cortical neurons sequentially in vitro. (A): Neurons in the early passage (1–3) cultures express deep layer markers Tbr1 (left) and Ctip2 (middle) but not Satb2; the superficial layer marker Satb2 first appeared after passage 3 (right). (B): Neurons in the RG cultures are positive for glutamatergic marker Vglut1. (C): Quantification and comparison of neuronal subtypes at different passages. Although the percentage of neurons (blue dotted line) remained constant through passage 5, most neurons in passage 1–3 were Tbr1- and Ctip2-positive. Satb2-positive neurons did not appear until passage 4. Data are mean ± SEM. Scale bars = 50 μm. Abbreviation: DAPI, 4′,6-diamidino-2-phenylindole.