Skip to main content
. 2015 Mar 30;4(5):523–531. doi: 10.5966/sctm.2014-0200

Figure 2.

Figure 2.

Stable expression of Atsttrin and GFP in MSCs. (A): Schematic map of the lentiviral vector. (B): mRNA expression levels of Atsttrin in MSC-GFP and MSC-Atsttrin cells by quantitative real-time polymerase chain reaction (qRT-PCR). (C): mRNA expression levels of GFP in MSC-GFP and MSC-Atsttrin cells by qRT-PCR. (D): Western blot analysis of MSC-GFP and MSC-Atsttrin cells with HA antibody. (E): Green fluorescent protein expressed by MSC-GFP. Scale bars = 20 µm. ∗∗∗, p < .001. Abbreviations: Col II, collagen II; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; HA, hemagglutinin; MSC, mesenchymal stem cell.