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. 2015 Mar 30;4(5):513–522. doi: 10.5966/sctm.2014-0267

Figure 6.

Figure 6.

HucMSC-Ex-delivered Wnt4 was critical for angiogenesis in vitro and in vivo. (A): HucMSCs were transfected with lentivirus Wnt4-shRNA or control-shRNA. The expression of Wnt4 in hucMSCs and hucMSC-Ex was determined using Western blot. (B): Cytoplasm and nuclear fractions were prepared from EA.hy926 cells treated with exosomes from Wnt4-shRNA or control-shRNA-transfected hucMSCs. β-catenin protein levels were determined using Western blot. (Ci–Ciii): The tube-formation ability of EA.hy926 cells treated with PBS, shWnt4-Ex, or shControl-Ex was determined (×40). (Civ): Measurement of the tube length by Image J software. ∗∗, p < .01; ∗∗∗, p < .001. (D): The rat wound models were treated with PBS, shControl-Ex, or shWnt4-Ex for 1 week. The expression of CD31 in the wound area was determined using immunofluorescence staining (×200). Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; hucMSCs, human umbilical cord mesenchymal stem cells; hucMSC-Ex, exosomes from human umbilical cord mesenchymal stem cells; n.s., not significant; PBS, phosphate-buffered saline; PCNA, proliferating cell nuclear antigen; shControl, control-short hairpin RNA lentiviral expression vector; shControl-Ex, exosomes from control-short hairpin RNA-transfected hucMSCs; shWnt4, Wnt4-short hairpin RNA lentiviral expression vector; shWnt4-Ex, exosomes from Wnt4-short hairpin RNA-transfected hucMSCs.