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. 2015 Mar 24;140(9):3090–3097. doi: 10.1039/c4an01899k

Fig. 3. The presence of large nanoparticles allows analysis of large target areas rapidly using StreamLine™ Raman. A large cell clump seen by light microscopy (A) was analysed to quickly give false colour image maps; (B + D) = intensity at 1295 cm–1 (CH2 deformations); and, (C + E) = signal to baseline at 1194–1228 cm–1 (Amide III).35 Areas of high intensity (red) appear to correspond to areas of high nanoparticle localisation. Also, by increasing laser power, regions of relevant high SERS expression become easier to determine (B + C = 0.05% laser power; D + E = 0.1% laser power). Scale bar = 20 μm.

Fig. 3