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. 2015 Apr 29;10(4):e0124093. doi: 10.1371/journal.pone.0124093

Fig 2. Specificity of PluR and PauR towards different signaling molecules.

Fig 2

PluR (A) senses its cognate signaling molecule PPYD, whereas unrelated signaling molecules, like C8-HSL or DAR, CHDA, CHDB and IPS, are not sensed. To test the specificity of PluR the reporter system pBAD24-His-pluR and pBBR1-pcfA P.l.-lux was used. Similarly, PauR (B) specifically senses its native signaling molecules with the highest specificity towards DAR compared to the DAR-precursors, CHDA, CHDB and IPS. The PauR-specific reporter plasmid system composed of pBAD24-His-pauR and pBBR1-pcfA P.a.-lux was used. Cells harboring the promoter-less reporter plasmid in combination with each PluR and PauR did not exhibit significant pcfA promoter activity. Furthermore, cells harboring the empty pBAD24 plasmid, and therefore no pluR or pauR, with the respective reporter plasmid as well did not exhibit significant pcfA promoter activity. RLUs are shown for 2 h after addition of the depicted signaling molecule. Reference line was set to 370 RLUs to underline the background of the system. RLU, relative light units. (C) Comparison of the structures of the signaling molecules used in this study.