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. 2015 Apr 29;10(4):e0124093. doi: 10.1371/journal.pone.0124093

Fig 4. The TYDQYI-motif in the SBD of PauR is essential for the overall functionality of the receptor and DAR-sensing.

Fig 4

The most drastic effects on DAR-sensing were gained with the replacement of S38A, T62A, Y66A, D75A and D75N in the SBD of PauR and a decreased effect on DAR-sensing were gained with the replacement of Y90C and I113S in PauR (lower right quadrant). The PauR derivatives Y40A, Y40F, D75E and Q76A dramatically influenced the structure of PauR and decrease its ability to bind and activate pcfA P.a. promoter (lower left quadrant). The activity of pcfA P.a. promoter was measured via luminescence as read-out and pictured values were taken 2 h after addition of 0.1% arabinose (lower axis) or 3.5 nM DAR (left axis) and compared to PauR wild type, which values were set to 100% (upper right quadrant). To evaluate the different derivatives, a cut-off of 70% was set for each value. RLU (relative light units) values for all PauR derivatives and PauR wild type are depicted in S4 Table.