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. 2014 Nov 25;11:54. doi: 10.1186/1743-7075-11-54

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© Ito et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ALDH1A1 mRNA expression and localization by in situ hybridization. Livers of VAA rats were used to detect rat ALDH1A1 mRNA expression (purple color). Green signals show nuclei counter stained by methyl green dye. Hepatocytes are notable by their large, round, methyl green-stained nuclei. Staining for ALDH1A1 mRNA was present throughout the parenchyma but was most intense around the portal tracts, including surrounding bile ducts (arrow, vehicle-treated liver). A sense RNA probe control is also illustrated. Magnification: × 200. Results for VAD liver were similar and therefore are not shown.