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. 2014 Nov 25;11:54. doi: 10.1186/1743-7075-11-54

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© Ito et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Co-localization of ALDH1A1 protein expression and rat macrophage marker ED1 in liver from VAA and VAD rats. Dual IHC with anti-ALDH1A1 antibody and anti-ED1 antibody was performed followed by methyl green counterstaining for detection of nuclei. Anti-ALDH1A1 staining (purple) showed a broad distribution, which was intense around portal regions and bile ducts, while cells staining for ED1 (pink-red) was more generally scattered in the parenchyma and in portal areas after treatment with LPS (Figure 4 e and f compared with a and b). Magnification: x400. Panel i shows negative staining control.