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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mol Microbiol. 2015 Jan 24;95(6):925–944. doi: 10.1111/mmi.12905

Table 2. Co-localization of FtsL or FtsL* with ZapA at the division site.

genotype % of total cells:
with stable ZapA-mCherry
ring at the division site		 with GFP-FtsL ring at
the division site		 with co-localized
proteins
WT zapA-mCherry
(Plac::gfp-ftsL) 		64.9 ± 1.1 38.1 ± 4.1 37.3 ± 4.4
ftsL* zapA-mCherry
(Plac::gfp-ftsL*) 		54.3 ± 1.4 41.8 ± 2.7 41.3 ± 2.5

Overnight cultures of TU211 (attHKMT35) [WT zapA-mCherry (Plac::gfp-ftsL)] and MT102(attHKMT36) [ftsL* zapA-mCherry (Plac::gfp-ftsL*)] were diluted in minimal M9 medium supplemented with 0.2% maltose and 25uM IPTG and grown at 30°C until an OD600 ~ 0.25 - 0.35. The cells were imaged using phase contrast, mCherry and GFP optics and analyzed using the imaging software NIS-Elements (Nikon) to determine the number of cells with a ZapA-mCherry ring or a GFP-FtsL(wt or L*) ring or both at the division site. 1200 cells were analyzed for each condition, n = 3. Shown are the average values ± standard deviation.