Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 29;11(4):e1005153. doi: 10.1371/journal.pgen.1005153

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Robledo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 5 — Morphological phenotype (A) and DNA content (B) of Rm4011ecpR1 overexpressing ecpR1-2 carrying 2 nt exchanges in the predicted interaction region. The bar represents 2 μm. (C, D) Predicted duplexes between EcpR1 and either gcrA or dnaA mRNAs. Numbers denote positions relative to the AUG start codon of the mRNA and the second 5’-end of EcpR1. The predicted energy score (E) is indicated in kcal/mol. The nucleotide exchanges in the mRNAs of gcrA (gcrA-BS-egfp) and dnaA (pdnaA-BSs-egfp) as well as in EcpR1 (EcpR1-2) are indicated in bold. (E, F) Fluorescence measurements of 4011ecpR1 co-transformed with ecpR1, ecpR1-2, or control SmelC812 overexpression plasmids and the indicated reporter plasmids. Reporter constructs carried either native mRNA sequences derived from gcrA or dnaA or variants with mutations in predicted EcpR1 binding sites (BS). Fragments are delineated in Fig 4. Reporter construct activities were determined as in Fig 4.