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. 2015 Apr 29;11(4):e1005153. doi: 10.1371/journal.pgen.1005153

Fig 6. Hfq and RNase E activities are dispensable for EcpR1 overproduction-related cell elongation and post-transcriptional repression of gcrA.

Fig 6

(A) Northern blot analysis of EcpR1 stability in Rm2011 and hfq mutant strains grown to early stationary phase (OD600 of 1.2, t = 0) and upon transcription arrest with Rf at indicated time points (in min). (B) Cell morphology of 2011hfq and 2011rne::Tn5 mutants overexpressing either ecpR1 (EcpR1+) or the control RNA gene SmelC812 (Control+) upon IPTG induction. Bars represent 2 μm. (C) Percentage of fluorescence in EcpR1 overproduction strains relative to the respective control strain overproducing SmelC812 in the Rm4011ecpR1 or Rm4011ecpR1 rne675 background co-transformed with plasmids carrying pPgcrA-gcrA-egfp or pdnaA-154+162-egfp translational fusions. (D) qRT-PCR analysis of gcrA and dnaA transcript abundance in Rm4011ecpR1 EcpR1+ after transcription arrest with Rifampicin for 5 minutes. Values were normalized to the SMc01852 transcript and the levels in the IPTG induced control strain overexpressing the SmelC812 RNA gene. Results from three independent experiments are shown. Error bars indicate the standard deviation.