Table 1. Sequences and annealing temperature of the primers used for the characterization of Lama glama casein genes cluster.

Gene	Region covered	Position	Primer	Sequence	Ta(°C)
CSN1S1	5’-flanking region	Promoter	Forward*	5’-GGGAATCTTATTGATGTAACAGT-3’	60.5
		Exon1	Reverse	5’-GGGAAGAAGCAGCAAACT-3’
	cDNA	Exon 1	Forward*	5’-AGTTTGCTGCTTCTTCCC-3’	61.0
		Exon 21	Reverse*	5’-ATGGCAGTTACAGGAGAAG -3’
	3’-flanking region	Exon 21	Forward	5’-AGTATGAAGGCCACCAAATA-3’	61.5
		3’-end	Reverse*	5’-CTCTGTTCCCACACCTTT-3’
CSN2	5’-flanking region	Promoter	Forward*	5’-GTTTCTCCATTACAGCATC-3’	60.0
		Intron 1	Reverse †	5’-TCAAATCTATACAGGCACTT-3’
	cDNA	Exon 1	Forward †	5’-TTCACTTCTTTTCCTCCAC-3’	62.0
		Exon 9	Reverse †	5’-TAGTTTATTGAAGTGACTGGT-3’
	3’-flanking region	Exon 9	Forward	5’-GGCACCTTTTCAATCTTT-3’	61.4
		3’-end	Reverse*	5’-TGTAATAGTTTAGCTGAGAT-3’
CSN1S2	5’-flanking region	Promoter	Forward*	5’-AACTAATATGAGAGCTGAG-3’	60.5
		Exon1	Reverse	5’-TGAAGGGAAGACAAGTA-3’
	cDNA	Exon 1	Forward*	5’- CACTGCCTGGACTACTT-3’	60.5
		Exon 17	Reverse*	5’- TCAAAGAGTAGTGAAAACATTTTC-3’
	3’-flanking region	Exon 17	Forward	5’-AGACTGTGCAGAATATTTCC-3’	60.3
		3’-end	Reverse*	5’-ATGTGAACTGGGAGGAG-3’
CSN3	5’-flanking region	Promoter	Forward #	5’-CACAAAGATGACTCTGCTATCG-3’	62.0
		Intron1	Reverse #	5’-AGAAGTTAGCCCTCCACAT-3’
	cDNA	Exon 1	Forward #	5’-GGCCAGCTCAACCTACT-3’	55.0
		Exon 6	Reverse #	5’-GCATTCAATTAGCTTTATTA -3’
	3’-flanking region	Exon 6	Forward	5’-GTCTTGCTGAAACCAAA-3’	56.5
		3’-end	Reverse*	5’-ATTCCCGAGAGCAATTT-3’

Asterisks refer to primers designed on ferus camel genome sequence available in gene bank (EMBL acc. no. AGVR01039100.1); crosses and hashes indicate primers designed on camel CSN2 (EMBL acc. No. HG969421) and CSN3 (EMBL acc. No. HE863813) sequences respectively, whereas the other primers were designed on newly determined llamas cDNA sequences.