Table 3. Most representative consensus motifs for transcription factors detected in the 5’-flanking regions of llamas by TFSEARCH software and present in all caseins with higher binding score (BS).
| Transcription factor | Consensus motif | CSN1S1 | CSN2 | CSN1S2 | CSN3 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Position | S | BS | Position | S | BS | Position | S | BS | Position | S | BS | ||
| AML1/Runx | TGTGGT | -259/-254 | - | 0.873 | -57/-52 | - | 1.000 | -300/-295 | + | 0.910 | -61/-56 | - | 0.850 |
| AP-1 | RSTGACTNMNW | -186/-176 | - | 0.850 | -98/-88 | + | 0.851 | -104/-94 | - | 0.890 | |||
| C/EBP | NNTKTGGWNANNN | -304/-292 | - | 0.940 | -271/-259 | + | 0.911 | -51/-39 | - | 0.927 | -58/-45 | - | 0.875 |
| GATA | NNNGATRNNN | -106/-97 | + | 0.870 | -183/-174 | + | 0.887 | -350/-341 | - | 0.860 | -124/-115 | - | 0.931 |
| HNF3 | NNNTRTTTRYTY | -83/-72 | + | 0.880 | -77/-66 | + | 0.928 | -338/-327 | + | 0.928 | -20/-9 | + | 0.932 |
| MyoD | SRACAGGTGKYG | -307/-296 | + | 0.874 | -265/-254 | + | 0.925 | -62/-51 | - | 0.858 | |||
| Oct-1 | NNNRTAATNANNN | -267/-255 | - | 0.929 | -131/-120 | + | 0.917 | -186/-174 | + | 0.947 | -84/-72 | + | 0.852 |
| Pbx-1 | ANCAATCAW | -45/-37 | + | 0.942 | -109/-101 | + | 0.903 | -221/-213 | + | 0.899 | -33/-25 | - | 0.912 |
| SRY | AAACWAM | -253/-248 | + | 0.941 | -192/-186 | - | 0.960 | -169/-163 | + | 0.947 | -14/-8 | - | 0.939 |
| MGF/STAT5 | TTCCCRKAA | -270/-262 | + | 0.931 | -94/-86 | - | 0.870 | -292/-284 | - | 0.956 | |||
| TATA-box | WTATAAAW | -31/-25 | + | 0.980 | -28/-19 | + | 0.910 | -23/-16 | + | 0.865 | -18/-11 | - | 0.927 |
DNA strands (S) in direction 5’>3’ are indicated by +.
The opposite strands are indicated by-.