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. 2015 Apr 29;11(4):e1004859. doi: 10.1371/journal.ppat.1004859

Fig 2. Expression of bdv-p or bdv-x gene does not alter hNPCs at the undifferentiated stage.

Fig 2

RNAs from bdv-p- and bdv-x-expressing hNPCs and their matched NT controls were analyzed by RT-qPCR for expression of (A) Nestin and (B) Sox2. Proliferation of bdv-p and bdv-x-expressing hNPCs was analyzed by BrdU labeling (C) and by a mitochondrial dehydrogenase activity-based assay (D and E) in the presence of growth factors and by enumeration of DAPI-positive cells (F and G) in the absence of growth factors. Results in A and B are representative of two independent experiments performed in triplicate. Results in C represent the mean of two independent experiments performed in quintuplicate. Results in D and E are representative of 2 independent experiments performed in quintuplicate. Results in F and G are from 1 experiment performed in triplicate. Statistical analyses were performed using the Mann-Whitney test. ns, non-significant (p > 0.5).