Fig 4. bdv-p expression does not induce cellular death in differentiated hNPCs.

Transduced hNPCs expressing bdv-p or bdv-x genes and their matched NT controls were induced to differentiate for 14 days and observed by (A) phase-contrast microscopy. Apoptosis was quantified based on (B) immunostaining with an antibody directed against cleaved-caspase 3 (green) and (C) TUNEL assay (green). Nuclei were stained with DAPI (blue). The data represent the mean of 2 independent experiments performed in triplicate. Statistical analyses were performed using the Mann-Whitney test. ns, non-significant (p > 0.5). Scale bar, 20 μm.