Fig 6. The S26/28 phosphorylation site is not necessary for bdv-p-induced reduction in GABAergic neurogenesis.

(A) Schematic representation of P and Paass. Paass is mutated in S26/28 residues and thus lacks the corresponding phosphorylation site. (B) hNPCs were transduced with lentiviral vectors bearing the bdv-p or bdv-p _aass gene and immunostained at the undifferentiated stage (day 0) with an antibody directed against the P protein (green). Nuclei were stained with DAPI (blue). Transduction efficiency was evaluated by enumeration of P-positive cells (right panel). (C) Western blot showing the level of P in bdv-p and bdv-p _aass-expressing hNPCs at 14 days of differentiation. P is normalized to actin. (D) Transgenic hNPCs and their NT matched controls were induced to differentiate for 14 days and GABAergic neurons were quantified. The results are representative of 2 independent experiments performed in triplicate. Statistical analyses were performed using the Mann-Whitney test. ***, p = 0.002. Scale bar, 20 μm.