Table 1.
Summary of the imaging sequence applied for sensitivity analysis
| Ref# | Imaging Parameters | ||||
|---|---|---|---|---|---|
| Start time a | Type | n-dir | b-value (s /mm 2 ) | Scan duration b | |
| 1 | 2:00 | DTI | 6 | 1000 | 1:50 |
| 2 | 3 :50 | DTI | 12 | 1000 | 3:56 |
| 3 | 7:46 | DTI | 6 | 1000 | 1:50 |
| 4 | 9:36 | DTI | 12 | 500 | 3:56 |
| 5 | 13:32 | DTI | 6 | 1000 | 1:50 |
| 6 | 15:22 | DTI | 12 | 2000 | 3:56 |
| 7 | 19 :18 | DTI | 6 | 1000 | 1:50 |
| 8 | 21:08 | T1W | NA | NA | 18:12 |
| 9 | 39:20 | DTI | 6 | 1000 | 1:50 |
| 10 | 41:10 | DTI | 12 | 1500 | 3:56 |
| 11 | 45:06 | DTI | 6 | 1000 | 1:50 |
| 12 | 46:57 | T1W | NA | NA | 18:12 |
| 13 | 65:09 | DTI | 6 | 1000 | 1:50 |
| 14 | 66:59 | DTI | 12 | 2500 | 3:56 |
| 15 | 70:55 | DTI | 6 | 1000 | 1:50 |
The first scan was carried out at 2 h and subsequent serial scans were carried out without moving the heart in the scanner. A baseline scan taking 1 h 50 m for 6 diffusion directions at b = 1000 s:mm2 was performed at serial time-points throughout the imaging study to explore any changes in diffusion as a result of time elapsed from killing/perfusion fixation. Interspersed between these baseline scans two T1W FLASH scans were performed at 50 × 50 × 50 μm resolution, as well as a series of diffusion scans with different numbers of directions and different b-values. All diffusion scans were at 200 × 200 × 200 μm resolution. Ref #: reference number for this scan; a– time post-fixation (h:min); bduration (h:min); Type: DTI - diffusion tensor magnetic resonance imaging; T1W is T1-weighted FLASH; FLASH: fast low angle shot; n-dir: number of diffusion directions; NA: not applicable.