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. 2015 Apr 14;12:59. doi: 10.1186/s12985-015-0292-6

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© Isok-Paas et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mapping of polyadenylation CSs in the HPV11 genome in U2OS cells via 3´ RACE. U2OS cells were transfected with the HPV11wt genome (A and B, lane 1) or together with the linearized pBabe-Neo construct (A and B, lanes 2 and 3). Mock transfection was used as a negative control (lane 4). PolyA⁺ mRNA was extracted at 4 or 10 days post-transfection. All distinct products were purified, cloned, sequenced and analyzed. (A) 3´ RACE analysis of the HPV11 early region CSs using the HPV11-specific primer pr3392. The indicated 3´ RACE products represent the potential CSs at nt 4384 and nt ~3248. (B) 3´ RACE analysis of the HPV11 late region CSs using the HPV11-specific primer pr7188. The indicated 3´ RACE products represent the potential late CSs at nt 7485, at nt ~7770 and at nt ~7587.