Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Phytother Res. 2015 Jan 30;29(4):582–590. doi: 10.1002/ptr.5291

Figure 1. Whole cell patch clamp analysis of kava extract-induced conductances in RBL2H3 cells.

Figure 1

RBL2H3 were analysed in the whole cell configuration with applied 1% aqueous kava extracts (Mahakea) prepared as described and applied in internal solution over the indicated time course. Three types of conductance were observed repeatedly in the cell population (n= 21 cells). Examples of each of these conductance types are shown here. A, C, E. Inward and outward current development over time. B, D, F. Current/voltage (I/V) relationships. A, B. Development of TRPV-like current after application of kava extract (n=7/21). C, D. Development of TRPM-like current after application of kava extract (n=2/21). E, F. Development of ICRAC-like current after application of kava extract (n=4/21). CRT; control. External solution: 20mM Cacl2, 2mM MgCl2, 130mM NaCl, 2.8mM CsCl, 10 mM NaOH-HEPES, 11mM Glucose. Internal solution: 140mM Cs-Glut, 8mM NaCl, 3mM MgCl2, 10 mM CsOH-HEPES, 10mM BAPTA, 4.3mM CaCl2.