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. 2015 Apr 29;10(4):e0123352. doi: 10.1371/journal.pone.0123352

Fig 3. Phosphorylation of endogenous eIF4E increases cellular resistance to stress.

Fig 3

A, Expression of phospho-eIF4E with and without CGP57380 treatment and with arsenite or cisplatin treatment in MDA-MB-231 cells. B, MDA-MB-231 cells treated with CGP57380 to inhibit Mnk1/2-mediated phosphorylation of eIF4E displayed slightly decreased cell proliferation under normal conditions, whereas cell recovery after oxidative stress by arsenite (and to less extent cisplatin) was dramatically reduced. C, Western blotting showed a lack of phospho-eIF4E in MEFs from Mnk1/2 null mice and significantly reduced phospho-eIF4E in wild-type MEFs treated with CGP57380. D, MTT growth assays demonstrated a slight decrease in the proliferation of MEFs from Mnk1/2 null mice compared to wild-type MEFs and a significant reduction in cell recovery following either arsenite or cisplatin treatment. E, Inhibiting Mnk1/2 in wild-type MEFs with CGP 57380 significantly reduced cellular recovery from arsenite or cisplatin treatment. * = P<0.05, ** = P<0.01 and *** P = <0.001 compared to control, n = 3.