Table 4. PUE candidate genes detected based on converging evidence from functional annotation, P-responsiveness based on gene expression profiling and SNP variation in the promoter and coding regions.
Chr. | MSU_LOC: | MSU_5' | MSU_3' | MSU_Annotation | Pariasca-Tanaka et al. (2010) | Zheng et al. (2009) | Number of NS-SNPs b | Number of P-SNPs c |
---|---|---|---|---|---|---|---|---|
1 | LOC_Os01g13030 | 7248761 | 7253629 | OsIAA3, Auxin-responsive Aux/IAA gene family member, expressed | 0.79** Sa | 0.74* S | 1 | 4 |
LOC_Os01g13060 | 7264228 | 7272237 | CK1_CaseinKinase_1.1—CK1, expressed | 0.53* S | NS | 1 | 5 | |
LOC_Os01g13080 | 7279250 | 7277720 | 60S acidic ribosomal protein, putative, expressed | 2.16*** S | 1.65* R | 0 | 2 | |
LOC_Os01g13090 | 7280734 | 7284992 | nucleic acid binding protein, putative, expressed | NS | NS | 4 | 8 | |
LOC_Os01g13150 | 7324145 | 7331536 | metallo-beta-lactamase family protein, putative, expressed | na | 1.94* R; 2.99* S | 14 | 16 | |
LOC_Os01g13160 | 7332691 | 7338244 | expressed protein | 0.81** R | NS | 4 | 2 | |
LOC_Os01g13170 | 7339717 | 7344465 | ubiquitin-conjugating enzyme E2, putative, expressed | NS | NS | 6 | 15 | |
4 | LOC_Os04g30110 | 17790725 | 17789889 | wall-associated receptor kinase 3 precursor, putative, expressed | na | 1.24*** S | ND | ND |
11 | LOC_Os11g29470 | 16634045 | 16631968 | expressed protein | na | na | 4 | 4 |
LOC_Os11g29490 | 16642596 | 16649451 | plasma membrane ATPase, putative, expressed | NS | NS | 2 (stop codon) | 2 | |
12 | LOC_Os12g42970 | 26659915 | 26662912 | GATA zinc finger domain containing protein, expressed | NS | 1.86* R | ND | ND |
12 | LOC_Os12g43110 | 26734618 | 26735291 | OsSAUR58, Auxin-responsive SAUR gene family member, expressed | NS | 1.8* S; 2.6** R | ND | ND |
aValues above 1 indicate up-regulation of the gene under P deficiency.
Asterisks indicate statistical significance, where * P<0.05, ** P<0.01, *** P<0.001, and NS indicates P>0.05.
(S) and (R) refer to shoot and root tissue, respectively. ‘na’ indicates that the data were not available.
bNS-SNP: non-synonymous SNP. Variation based on information taken from RiceVarMap, http://ricevarmap.ncpgr.cn/.
cP-SNP: SNP within a 1 kb upstream promoter region. Variation based on information taken from RiceVarMap, http://ricevarmap.ncpgr.cn/.