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. 2015 Apr 29;10(4):e0124743. doi: 10.1371/journal.pone.0124743

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© 2015 Yoshihama et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — Fluorescently labeled h1299/zeo or h1299/CD82 cells (4.0 × 10⁴ cells/well) were applied to a HUVEC monolayer culture and allowed to adhere at 37°C. Adhered cells were quantified after 30 min. Adhered h1299/zeo (A) and h1299/CD82 (B) cells and the effect of function-perturbing antibodies on cell adhesion to the HUVEC monolayer were analysed (C). h1299 cells were pre-treated with 5 μg/ml of the indicated antibodies and then applied to the HUVEC monolayer. Experiments were performed in triplicate and the number of adhered cells was averaged. Bars indicate the standard deviation.