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. Author manuscript; available in PMC: 2016 Jun 5.
Published in final edited form as: Chem Biol Interact. 2014 Nov 4;234:144–153. doi: 10.1016/j.cbi.2014.10.029

Fig. 5.

Fig. 5

15-oxoETE inhibits NF-κB activity. (A) HEK293T cells stably expressing a 5x-κB driven firefly luciferase reporter were pre-treated with or without 100 µM of the GST inhibitor ethacrynic acid for 1 hr then treated with 15-oxoETE for 5 min at increasing doses followed by TNFα (40 ng/mL) for 6 hr. Luciferase activity was determined in a luminometer then normalized to the vehicle (0.25% DMSO) treated control. (B) Recombinant human IKKβ was incubated with increasing concentrations of 15-oxoETE or wedelolactone (100 µM), followed by addition of biotin tagged substrate (IκBα) and ATP.