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Rate constants determined from transient multiple turnover and steady-state experiments (5, 6).
| Parameters | kburst (s−1) | kss (s−1) |
|---|---|---|
|
Burst kinetics
|
||
| aldosterone | 1.98 ± 0.05 | 0.094 ± 0.002 |
| cortisone | 1.39 ± 0.04 | 0.104 ± 0.002 |
| testosterone | 0.71 ± 0.03 | 0.050 ± 0.002 |
|
Linear kinetics
|
||
| 7α-hydroxycholestenone | NA | 0.0160 ± 0.0002 |
| cholestenone | NA | 0.0060 ± 0.0002 |
Experiments were performed at 37 °C in 100 mM potassium phosphate buffer (pH 6.0), containing 4% acetonitrile as cosolvent.
