Fig. 3.

Oxidation of DEAB in the absence of enzyme. Wavelength scans indicate that DEAB oxidizes to its acid at a slow rate in a 50 mM Na⁺-BES pH 7.5 at 25 °C, the buffer conditions used for wavelength scn assays with the enzymes. No NAD⁺ is present. The green line represents a water trace while the magenta line is BES alone (no DEAB). Over time, there was a decrease at 360 nm corresponding to DEAB and an increase at 300 nm corresponding to diethylaminobenzaldehyde. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)