Table 4.
Correlation of alcohol dehydrogenase activity and elimination of alcohols in rats
| Alcohol | in vitroa
v rel |
in vivob
mmole/kg/h |
Rate due to ADHc |
%ADHc |
|---|---|---|---|---|
| Ethanol | 0.88 | 7.9 ± 1.0 | 7.0 | 89 |
| 1-Propanol | 0.91 | 5.3 ± 0.5 | 4.3 | 81 |
| 1-Butanol | 1.3 | 3.2 ± 0.4 | 1.8 | 56 |
| 2-Methyl-1-propanol | 0.77 | 3.8 ± 0.5 | 2.5 | 66 |
| 2-Methyl-1-butanol | 0.53 | 4.6 ± 0.8 | 2.8 | 61 |
| 2-Propanol | 0.049 | 1.1 ± 0.1 | 0.6 | 55 |
| 2-Propanol-d7 | 0.011 | 0.55 ± 0.04 | N.D. | N.D. |
| 2-Butanol | 0.14 | 2.5 ± 0.4 | 1.7 | 68 |
| 3-Pentanol | 0.35 | 4.0 ± 1.0 | 3.6 | 90 |
| Cyclopentanol | 0.68 | 4.7 ± 3.2 | 3.0 | 64 |
| Cyclohexanol | 0.90 | 4.3 ± 1.5 | 3.2 | 74 |
| 1,3-Propanediol | 0.13 | 2.7 ± 0.8 | 1.7 | 63 |
| 1,3-Butanediol | 0.44 | 5.0 ± 0.3 | 4.1 | 82 |
| 1,4-Butanediol | 0.52 | 6.7 ± 0.1 | 5.8 | 86 |
a
Calculated from kinetic constants, v rel = Vrel(5)/Km + 5) for 5 mM alcohol.
b
Data for zero order elimination; or for first order elimination, where rate = k1(min−1 • (5 mmole/L)•r(L/kg)•60min/h).
c
Estimated from the observed rate without inhibitors minus the rate with 1 mmole/kg 4-methylpyrazole. “%ADH” is the estimated percentage of elimination due to ADH.