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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cell Tissue Res. 2015 Jan 24;360(2):321–332. doi: 10.1007/s00441-014-2088-1

Figure 2.

Figure 2

(a–b”) MDCK cells transfected with the TGN-marker enzyme galactosyltransferase-CFP (a), fixed and labeled for GOPC (a’). b-b” show the inset region in image a”. There is extensive overlap of the markers (b-b”, arrowheads). (c–d”) Cells double-labeled with antibodies against the trans-Golgi cisternae marker Golgi-58K (c) and GOPC (c’). d-d” show the inset area in image c”. The labeling is in adjacent cisternae (d-d”, arrows). (e–f”) Cells double-labeled with antibodies against the medial Golgi marker GM130 (e) and GOPC (e’). f-f” show the inset area in image e”. The merged image shows no overlap of the markers (f-f”, arrows). Scale bar top panel, 20µm, bottom panel, 1µm. Representative images are from at least 4 experiments.