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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cell Tissue Res. 2015 Jan 24;360(2):321–332. doi: 10.1007/s00441-014-2088-1

Figure 3.

Figure 3

MDCK cells transfected with (a) Rab5-GFP, (b) Rab14-GFP, or (d) Rab11-GFP were fixed and labeled for GOPC (a’, b’, c’, d’). There is colocalization of GOPC with Rab5-GFP (a-a”, arrowheads) and Rab14-GFP (b-b”, arrowheads). Treatment of cells with Brefeldin A results in tubulation of Rab14 (c). However, GOPC distribution is unaffected (c’). (d-d”) There is no overlap of GOPC with Rab11-GFP. e-e” show the inset in image d”. Scale bars, top panels, 20µm, bottom panel, 4µm. Representative images are from at least 3 experiments.