Effect of AR allele strength on ETV1-induced PIN. a C57BL/6 mice carrying a “humanized” AR gene with a short (hAR
12Q) or long (hAR
48Q) polyglutamine tract were crossed with ETV1-transgenic FVB mice (ETV1
Tg) to generate hAR;ETV1
Tg mice. Experimental mice were castrated or left intact at 12 weeks, aged to 24 weeks and compared to intact hAR controls. hAR;ETV1
Tg mice were then backcrossed to FVB and crossed with Pten
+/− mice to generate hAR;ETV1
Tg
;Pten
+/− experimental mice with short (12Q), median (21Q) or long (48Q) Q-tracts. Experimental hAR;ETV1
Tg
;Pten
+/− mice were castrated or left intact at 12 weeks, aged to an average of 43 weeks and compared to hAR;Pten
+/− controls. b Representative H&E stained prostates from hAR;ETV1
Tg mice. Normal tissue (DLP), hyperplasia (DLP), and mPIN (VP) are shown. Images are ×10 with ×40 insets. c The proportion of mice with hyperplasia and mPIN in AP, DLP, and VP among hAR
12Q
;ETV1
Tg and hAR
48Q
;ETV1
Tg mice. Analysis was of 5-8 lobes per group