Fig. 7.

ETV1 overexpression alters genome-wide expression in Pten-hemizygous mice. a Principal component analysis was performed for all genes among the individual RNA-seq libraries. hAR ^12Q, hAR ^21Q, and hAR ^48Q mice are represented as triangles, squares, and circles, respectively. Green and red represent intact non-transgenic and transgenic mice, yellow and blue represent castrated non-transgenic and transgenic mice, and orange represents macroscopic tumors, respectively. b RNA-seq analysis was performed on DLP RNA as before. Heatmaps include differentially expressed genes between the groups shown (FDR ≤ 0.05), with columns representing the average among biological replicate libraries. By GLM analysis of all hAR;ETV1 ^Tg ;Pten ^+/− libraries vs. all hAR;Pten ^+/− libraries, 59 genes are upregulated and 3 downregulated in prostates of hAR;ETV1 ^Tg ;Pten ^+/− mice. Select significantly enriched GO terms are listed. c ETV1-upregulated genes shared among Pten ^+/− and Pten ^+/+ mice are listed. d Differential expression was performed between transgenics and non-transgenics for each AR Q-tract group individually. Overlap between genes differentially expressed in hAR ^12Q ;ETV1 ^Tg, hAR ^21Q ;ETV1 ^Tg or hAR ^48Q ;ETV1 ^Tg relative to non-transgenics is illustrated by Venn diagram